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. Author manuscript; available in PMC: 2016 Apr 21.
Published in final edited form as: Immunity. 2015 Apr 21;42(4):719–730. doi: 10.1016/j.immuni.2015.03.015

Figure 4. CD11c+ cell depletion reduces stromal expression of lymphocyte survival factors.

Figure 4

Mice were immunized on day 0 and treated or examined at indicated time points after immunization. (A) B cell BAFF receptor expression in Cd11c−DTRRag1−/− chimeras given PBS or DT at day 8 and examined at day 9. Representative of 3 pairs of mice from 2 experiments. (B–C) Expression of BAFF, IL-6, and IL-7 by (B) vascular-stromal and (C) CD11c+ cell subsets. Indicated populations were sorted from (B) day 9 or (C) day 8 draining nodes and cytokine expression evaluated by qPCR. “Whole lymph node” indicates collagenase-digested lymph node cells prior to cell separation. Note differences in scale in (B) and (C). Data pooled from (B) 4 and (C) 3 independent experiments. (D) Cytokine expression by PDPN+ reticular cells at day 9 after CD11c+ cell depletion at day 8 in CD11c-DTR mice. Data pooled from 3 independent experiments. For (B–D), each symbol represents 1 sample sorted from (B, D) 6–8 mice or (C) 4–5 mice. *=p<.05 and **-p<.01 using unpaired t-test compared to controls or as indicated; error bars are SD.