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. Author manuscript; available in PMC: 2016 Apr 21.
Published in final edited form as: Immunity. 2015 Apr 21;42(4):719–730. doi: 10.1016/j.immuni.2015.03.015

Figure 7. Disrupted immune responses with DC depletion can be rescued by survival factor administration or LTβR stimulation.

Figure 7

(A–B) BAFF and IL-7 administration. zDC-DTR chimeras were given PBS or DT at day 8 after immunization and indicated cytokine at 5–8 hours after PBS or DT. Mice were examined 20–24 hours after cytokine injection. The numbers of cells in indicated populations were normalized and compared to “DT+PBS” numbers. (A) Total, B, and T cell numbers. (B) Germinal center B cell and AFC numbers. Data from 4 experiments. (C–F) LTβR stimulation. Same experimental setup as in (A–B), except that zDC-DTR chimeras received control goat Ig or agonist anti-LTβR after DT. The numbers of indicated populations were normalized and compared to “DT+goat Ig” numbers. (C) PDPN+ reticular cells. (D) Total, B, and T cells. (E) Germinal center B cells and AFCs. (F) CD11c+ cell subsets. Data from 4 experiments. For (A–F), each symbol represents 1 mouse. *=p<.05 and **=p<.01 when compared to indicated controls using unpaired t-test; error bars are SD. See also Figure S7.