Table 1.
Histone modifications and associated MRM transitions used for quantification
| Histone | Sequence | Precursor ion (m/z) | Product ion (m/z) |
|---|---|---|---|
| H2A | AGLQFPVGR | 472.796 (2+) | 703.39 (1+, y6) |
| H2A (canonical) | VTIAQGGVLPNIQAVLLPK | 966.08 (2+) | 1092.68 (1+, y10) |
| H2A (1-A, 2-B, H2Ax) | LLGGVTIAQGGVLPNIQAVLLPK | 757.80 (3+) | 546.84 (2+, y10) |
| H2B | EIQTAVR | 408.73 (2+) | 446.27 (1+, y4) |
| H2B, K108-methylation | LLLPGELAKme | 484.31 (2+) | 736.46 (1+, b7) |
| H2B, K120-ubiquitination | AVTKubYTSSK | 549.79 (2+) | 827.43 (1+, y6) |
| H3 | DIQLAR | 358.21 (2+) | 359.24 (1+, y3) |
| H3, K4-, K9-acetylation | KacQLATKacAAR | 535.82 (2+) | 772.47 (1+, y7) |
| H4 | VFLENVIR | 495.29 (2+) | 501.31 (1+, y4) |
| H4, K8-, K12-, K16-acetylation | GGKacGLGKacGGAKacR | 606.34 (2+) | 927.54 (1+, y9) |
| H4, K12-, K16-acetylation | GLGKacGGAKacR | 464.27 (2+) | 530.30 (1+, y5) |
| H4, R55-methylation | ISGLIYEETRme | 597.82 (2+) | 290.18 (1+, y2) |
Modifications (ac) acetylation, (me) methylation, and (ub) ubiquitination of peptides were measured and normalized to an unmodified peptide, shown in italics, from the respective histone