Table 4.
BoNT/B LC mutants that result in loss of mAb binding. mAbs are grouped (separated by a solid line) based on shared amino acids. Amino acids indicated in bold were shared by the epitopes of more than one mAb.
| Epitope | mAb | Amino Acids Affecting mAb Binding |
|---|---|---|
| Epitope cluster I group 1 | 16B3 | G119, D120, Q177, N178, H179, R183, G185, I189 |
| 18E5 | Y117, G119, R121, R122, I132, A133, Q177, H179 | |
| 1B10.1 | R121, H179, D244 | |
| 2B25.1 | Y117, G119, E127, C308, I309 | |
| 1B22 | E127, P312, I309, I314, N315 | |
| 31A5 | G188, G185, P124, V135, H236, A133, I309 | |
| 19D22 | E22, A26, R31, P55 | |
| Epitope cluster I group 2 | 18A7 | R31, P55, T501 |
| 18D10 | R31, P55, T501 | |
| 31H3 | R31, R48, P55, F58 | |
| Epitope cluster I group3 | B6.1 | N81, K84 |
| 18A6 | N303, L306, V307, K320, D332, E334, K336, S338 | |
| Epitope cluster II | 4B19 | N6, N8, N10, D11, P12 |
| 19A9 | T392, I393, N396, S400, K402, D403, R409, Q411, V416 | |
| Epitope cluster III | 18F2 | K285, V341, E342K, D345, L347 |
| 31G2 | V341, S343, K346, K349 | |
| 31E2 | L238, F295, K326, G237, K346, K349 |