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. 2015 Sep 10;7(9):3572–3607. doi: 10.3390/toxins7093572

Figure 6.

Figure 6

A clade of nsc-like clusters in Trichophyton, a clade of vrt-like clusters in Metarhizium, and other closely related clusters. An excerpt of the group V phylogenetic tree made with FastTree [60], Figure S2, containing the PKSs from the neosartoricin-producing cluster, NscA (EAL84875) [49], and the viridicatumtoxin-producing cluster, VrtA (ADI24926) [48], and groups of related uncharacterized PKSs, primarily in Trichophyton and Metarhizium, respectively, is shown at top left. The bootstrap values are presented next to their corresponding nodes. The green boxes indicate PKSs from the same species in which the characterized clusters were originally described. Next to the tree are the gene clusters corresponding to the PKSs that were identifiable through MultiGeneBLAST analysis. Gene cluster diagrams next to brackets depict the cluster corresponding to the PKS with its accession number highlighted in red, but all of the bracketed PKSs belong to clusters which are identical in terms of the presence and synteny of their nsc- or vrt-cluster homologs. Genes are represented as arrows with a color corresponding to the proteins they encode which are detailed in the color key below the tree and cluster diagrams. Genes with no color were not identified as homologous to any group V2 cluster gene. The products of the characterized examples from this clade, neosartoricin and viridicatumtoxin, are shown at bottom. C6TF = GAL4-like Zn(II)2Cys6-domain and fungal-specific transcription factor domain-containing protein, VrtR1-like, MFS = Major Facilitator Superfamily transporter, P450 = Cytochrome P450, TA = Threonine aldolase, OGO = 2-oxoglutarate-Fe(II)-type oxidoreductase, PKS = Polyketide synthase, ACS = Acetoacetyl-CoA synthase, PPT = Polycyclic prenyltransferase, IDS = Isoprenyl diphosphate synthase, MT = Methyltransferase, MβL = Metallo-β-lactamase-type thioesterase, FMO = Flavin-dependent monooxygenase, TF = GAL4-like Zn(II)2Cys6-domain and fungal-specific transcription factor domain-containing protein, VrtR2-like, NDH = NAD-dependent dehydratase, LIP = Secretory lipase, ART = Arrestin, CNR = Copper-containing nitrite reductase, MGT = Magnesium transporter, ABCT = ABC transporter, Abr2 = Conidial pigment laccase, OMT = O-methyltransferase, EthD = EthD domain-containing protein, putative decarboxylase, PksP = Conidial pigment polyketide synthase.