Table 2.
Total isolation frequencies (Fr%) of Aspergillus section Aspergillus spp. and those of the four identified species from the animal feeds and the primary raw materials analysed. The Fr% of the unidentified section Aspergillus spp. includes isolates which could not be identified with certainty based on their growth morphological characteristics according to the identification key of Pitt and Hocking [8]. The proportion of A. proliferans among A. ruber isolates was estimated based on their restricted growth on CY20S [42].
| Animal Feed/ Raw Material | Fr% | |||||
|---|---|---|---|---|---|---|
| Total | A. montevidensis | A. chevalieri | A. pseudoglaucus | A. ruber (A. proliferans) | Unidentified Aspergillus spp. | |
| Rabbit feed * | 61.9 | 19.1 | 23.8 | 33.4 | 14.3 (1/7) | 4.8 |
| Chinchilla feed ** | 60 | 44 | 12 | 12 | 16 (5/7) | 12 |
| Rainbow trout feed *** | 25 | ND | ND | 17.9 | 14.3 (7/7) | 3.6 |
| Pelleted alfalfa (p) | 100 | 100 | ND | ND | 100 + | ND |
| Wheat bran (p) | 100 | ND | 100 | ND | ND | ND |
| Wheat millrun (c) | 100 | 100 | ND | ND | ND | ND |
| Pelleted soybeans (p) | 100 | ND | 100 | 100+ | ND | DN |
| Corn seeds (p) | 100 | ND | ND | ND | ND | 100 |
* data based on 21 analysed samples presenting three different feed formulations, ** data based on 25 analysed samples presenting three different feed formulations, *** data based on 28 analysed samples presenting two different feed formulations. One sample of each raw material type was analysed. (p) producer’s own preparation, (c) commercial product. ND, not detected. + The initial isolate identification was corrected after SEM and DNA analyses.