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. 2015 Oct 1;26(19):3424–3438. doi: 10.1091/mbc.E15-02-0113

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© 2015 Carvalhal et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — ALADIN localizes around the mitotic spindle and at the spindle poles in Drosophila and human cells. (A) Drosophila S2 cells expressing GFP-ALADIN and mCherry–α-tubulin in interphase (top) or metaphase (bottom). (B) Representative images of interphase (top) and metaphase (bottom) HeLa cells stably expressing GFP-ALADIN and transiently transfected with mCherry–α-tubulin. (C) GFP-ALADIN partially colocalizes with calnexin, an integral ER protein. (D) HeLa cells were fixed in the presence or absence of nocodazole and then stained to visualize tubulin and ALADIN. Scale bars, 10 μm.