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. 2015 Oct 1;26(19):3424–3438. doi: 10.1091/mbc.E15-02-0113

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© 2015 Carvalhal et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — ALADIN depletion does not affect the targeting of multiple centromere and kinetochore proteins or the kinases Plk1 and Aurora B. (A–C) Representative Z-stack images of HeLa cells treated with control and ALADIN siRNAs that were fixed and labeled for microtubules (anti-tubulin, green), kinetochores (ACA, white) and the indicated proteins (red). (D) Representative live-cell images of control and ALADIN-depleted HeLa cells stably expressing MCAK-mCherry and GFP-CENP-A. (E, F) Representative Z-series images of cells treated with control or ALADIN-specific siRNAs and fixed and stained with antibodies that recognize tubulin, ACA, and Plk1 or Aurora B. Scale bars, 5 μm.