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. 2015 Oct 1;8:525. doi: 10.1186/s13104-015-1474-4

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© Cantarella and D'Agostino. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The microsatellite region (36 nucleotides in length) in the reference sequence is shown in bold. PSR selects and counts only reads aligning the entire SSR region, in addition to one or more nucleotides to the left and right side different from bases in the repetitive units. Reads in grey are discarded since they partially cover the microsatellite. Reads in red are filtered out due to the uncertainty at the sequence borders. For each sample the program returns the number of repetitive units presenting coverage greater than the selected threshold (−p) in a tab delimited file