Effect of miRNA-107 on CLOCK mRNA and protein levels. Caco-2 cells were transfected with anti-miRNA-107 (50 nM), syn-miRNA-107 (5 nM), anti-miRNA-C (50 nM) or MRA-1001_miRHuman_17.xl syn-miRNA-C (5 nM) for 48 hours. (A) RT-qPCR of CLOCK, CRY1, CRY2, PER2 and PER3 after miRNA-107 inhibition. (B) RT-qPCR of CLOCK, CRY1, CRY2, PER2 and PER3 after miRNA-107 overexpression. Data are the mean of three independent experiments, each one performed in triplicate ± SEM (C) Analyses of CLOCK protein levels by Western Blot. Data are the mean ± SEM of ≥ 2 experiments performed in triplicate. * p<0.05; ** p<0.001.