TABLE 2.
Impact of deletion of MSH2 and RAD27 on rates of his7-2 mutations
The mutant strains are isogenic to E134 (wild type) and were obtained by dissection of tetrads of MSH2/msh2Δ RAD27/rad27Δ diploids. 95% confidence intervals are in parentheses, and the relative rates of 1-bp insertions are in braces.
| Genotype |
his7-2 mutation rate |
Rates of indicated his7-2 mutations (×10−8) |
|||
|---|---|---|---|---|---|
| Absolute mutation rate (×10−8) | Relative rate | 1-bp insertions in the A7 run (A7→A8) | Complex mutationsa | Other +1 frameshifts | |
| Wild typeb (n = 42) | 0.6 (0.5–1.2) | 1 | 0.5 {1} | 0.03 | 0.06 |
| msh2Δ (n = 41) | 120 (88–150) | 200 | 120 {240} | <3 | <3 |
| rad27Δ (n = 39) | 82 (66–110) | 140 | 48 {96} | 27 | 6 |
| msh2Δ rad27Δ (n = 41) | 6700 (5900–9400) | 11,000 | 6700 {13,400} | <160 | <160 |
a Each of the complex mutations consisted of an insertion and four or more other genetic alterations, all located within an ∼20-bp DNA segment.
b The wild-type data are from a previous report (42).