TABLE 3.
Effect of deletion of MSH2 and RAD27 on his7-2 mutation rate in the diploid S. cerevisiae
The mutant diploid strains are derivatives of FKY1037 (wild type) and were prepared using the lithium acetate/PEG/DMSO transformation method. CI, confidence interval.
| Genotype |
his7-2 mutation rate |
||
|---|---|---|---|
| Absolute rate (×10−8) | 95% CI | Relative rate | |
| Wild type | 0.9 | 0.7–1.3 | 1 |
| RAD27/rad27Δ MSH2/msh2Δ | 1.1 | 0.9–1.5 | 1 |
| RAD27/RAD27 msh2Δ/msh2Δ | 160 | 140–210 | 180 |
| rad27Δ/rad27Δ MSH2/MSH2 | 200 | 150–230 | 220 |
| RAD27/rad27Δ msh2Δ/msh2Δ | 150 | 30–190 | 160 |
| rad27Δ/rad27Δ MSH2/msh2Δ | 610 | 320–860 | 680 |
| rad27Δ/rad27Δ msh2Δ/msh2Δ | 13,000 | 11,000–16,000 | 14,000 |