TABLE 5.
Effects of the different mutant combinations on his7-2 mutation rate
The mutant haploid strains are isogenic to FKY688 (wild type) and were constructed using the lithium acetate/PEG/DMSO transformation method. 95% confidence intervals are in parentheses. The superscript italic letters a–e indicate mutation rates analyzed by Mann-Whitney U two-tailed test. The two mutation rates marked a do not statistically differ from each other (p = 0.15). The difference between two mutation rates labeled with the same letter (b–d or e) is statistically significant (b, p = 0.008; c, p = 0.012; d, p = 0.01; and e, p = 0.021).
| Genotype |
his7-2 mutation rate |
|
|---|---|---|
| Absolute rate (×10−8) | Relative rate | |
| Wild type | 0.7 (0.5–0.9) | 1 |
| msh3Δ | 2.3 (1.6–3.1) | 3 |
| msh6Δ | 3.0 (2.2–3.8) | 4 |
| msh3Δ msh6Δ | 110a (93–140) | 160 |
| msh2Δ | 140a (100–260) | 200 |
| rad27Δ | 46 (41–55) | 66 |
| msh2Δ rad27Δ | 6800b,c (4500–9900) | 9700 |
| msh3Δ msh6Δ rad27Δ | 6100d,e (4500–8800) | 8800 |
| msh3Δ rad27Δ | 91 (71–94) | 130 |
| msh6Δ rad27Δ | 520 (450–660) | 740 |
| mlh1Δ | 100 (88–130) | 150 |
| mlh1Δ rad27Δ | 3500b,d (2500–5100) | 5000 |
| pms1Δ | 100 (72–120) | 140 |
| pms1Δ rad27Δ | 3800 (2500–4300) | 5500 |
| pms1-E707K | 150 (110–230) | 210 |
| pms1-E707K rad27Δ | 3900c,e (2600–5100) | 5500 |