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Superimposition of Ran·Gpp(NH)p·RanBD1 (PDB code 1RRP, light green) and RanΔ191·GDP·BeF3·RanBD1 (this work, PDB code 5CLL, cyan). The side chains of Thr-24, Tyr-39, Thr-42, and Gln-69 and the nucleotides and BeF3 are shown as sticks, the magnesium atoms as spheres. The distance between the two magnesium positions is approximately 1.9 Å, and the light green magnesium ion of PDB code 1RRP occupies the position of a water molecule in RanΔ191·GDP·BeF3·RanBD1 (not shown for clarity).
