FIGURE 7.

SSB does not abolish M. tuberculosis RecG or RuvAB mediated fork reversal of leading strand gap structures. A, role of MtSSB and EcSSB proteins in the MtRecG- and MtRuvAB-mediated fork reversal of leading strand gap substrates. Reaction mixture contained 10 nm ³²P-labeled substrate in the presence of 1 mm ATP and 5 mm MgCl₂. Reactions were carried out either in the presence or absence of indicated proteins, and the products were resolved on 8% polyacrylamide gel and analyzed by autoradiography. Lanes 15 and 16 represent markers. B, quantitative data for the reactions shown in A. The error bars represent standard error of the mean (S.E.) from three independent experiments.