TABLE 3.

Catalytic parameters of ADP-bound tobacco Rca derived from global curve fitting procedures

Experiments were carried out at 5 μm Rca and 5 mm free Mg²⁺. The data sets were fit to Equation 2 with a fixed K_m value of 0.104 mm. The K_i value for ADP was fit globally, whereas k_cat and n_H values were fit individually for each ADP concentration. Indicated errors were estimated by the curve fitting algorithm.

[ADP]	Km	Ki	kcat	nH
μm	mm	mm	min−1
18	0.104 mm	0.037 ± 0.007	12.9 ± 0.5	1.2 ± 0.1
42			14.0 ± 0.5	1.5 ± 0.1
60			18.8 ± 0.6	1.6 ± 0.1
90			23.9 ± 0.6	1.8 ± 0.2
112			31.7 ± 0.8	1.8 ± 0.1
132			18.7 ± 0.8	1.9 ± 0.2