Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Aug 25;290(40):24237–24254. doi: 10.1074/jbc.M115.666883

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 8. — TUDC and cAMP induce serine dephosphorylation of Ntcp in HepG2-Ntcp cells. Ntcp-transfected HepG2 cells were stimulated with either TUDC (100 μmol/liter), Bt₂cAMP (10 μmol/liter) under normo-osmotic (305 mosmol/liter) conditions, or with hyperosmotic medium (405 mosmol/liter) without and with TUDC. GFP-tagged Ntcp was analyzed with regard to serine phosphorylation. Treatment with TUDC as well as treatment with Bt₂cAMP resulted in decreased serine phosphorylation, although hyperosmolarity triggered an increase in serine phosphorylation of the Ntcp. Ntcp serine phosphorylation was analyzed by Western blot (WB) and subsequent densitometric analysis. GFP served as a loading control. Ntcp serine phosphorylation under control condition was set to 100%. Data represent means ± S.E. of at least five independent experiments. IP, immunoprecipitation. *, p < 0.05 statistically significant compared with the unstimulated control. #, p < 0.05 statistical significance between hyperosmolarity and hyperosmolarity plus TUDC.