FIGURE 2.

Fractionation of WT and oeLHCSR1 solubilized thylakoids of N. benthamiana and N. tabacum. A, sucrose gradient of thylakoids from WT and oeLHCSR1 of N. benthamiana and N. tabacum plants solubilized with 0.8% α-DM. B, Coomassie Blue-stained SDS-PAGE analysis of green bands collected from the sucrose gradient. An equal volume (50 μl) of each band was loaded. A polypeptide corresponding to the molecular weight of LHCSR1, underlined in red, was clearly visible in B1 and B2 (corresponding to free pigments and monomeric LHCs, respectively). C, immunoblot analysis with α-PpLHCSR antibody. Thylakoids (Thyl) from oeLHCSR1 and WT were loaded as positive and negative controls, respectively. B1, B2, and B3 from WT thylakoids were loaded as additional negative controls.