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. 2015 Aug 20;290(40):24367–24380. doi: 10.1074/jbc.M115.666461

FIGURE 1.

FIGURE 1.

Mef2d shRNA adenovirus efficiently knocks down MEF2D. A, quantitative reverse transcriptase-PCR analysis of endogenous Mef2 knockdown in NRVMs 72 h post-transduction (day 3). The Mef2d shRNA adenovirus specifically knocks down Mef2d, without depleting Mef2a, Mef2b, or Mef2c. B, Western blot analysis of endogenous MEF2D knockdown in NRVMs and densitometry (C). shMef2d adenovirus efficiently knocks down MEF2D at both the transcript and protein level. The data are means ± S.E. (error bars). *, p < 0.05; ***, p < 0.001.