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. 2015 Aug 20;290(40):24367–24380. doi: 10.1074/jbc.M115.666461

FIGURE 2.

FIGURE 2.

Microarray validation in NRVMs. A, summary of a subset of cell cycle genes dysregulated by 1.5-fold or more in MEF2D depleted NRVMs. The majority of the positive cell cycle genes are up-regulated. Cdt1, chromatin licensing and DNA replication factor 1; Ccne1, cyclin E1; Ccne2, cyclin E2; Mcm3, minichromosome maintenance complex component 3; Mcm5, minichromosome maintenance complex component 5; Mcm5, minichromosome maintenance complex component 6. B, quantitative RT-PCR analysis of cell cycle regulatory genes dysregulated in MEF2D-depleted NRVMs at day 3. Pcna, proliferating cell nuclear antigen. C, Western blot analysis of cell cycle-associated proteins cyclin D1, cyclin D3, and cyclin-dependent kinase 2 (CDK2). D, Western blot densitometry. The data are means ± S.E. (error bars). *, p < 0.05; **, p < 0.01; ***, p < 0.001.