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. 2015 Aug 20;290(40):24367–24380. doi: 10.1074/jbc.M115.666461

FIGURE 3.

FIGURE 3.

Analysis of DNA synthesis, proliferation, and cell size in MEF2D-depleted cardiomyocytes. MEF2D-depleted NRVMs were cultured for 3 days post-transduction, followed by morphological analysis. A, MEF2D-depleted NRVMs showed no significant DNA synthesis by EdU incorporation 3 days post-transduction. B, MEF2D depleted NRVMs showed no significant proliferation as examined by Ki67 immunoreactivity. C, flow cytometric measurement of DNA content using propidium iodide (PI) staining shows a significant reduction in the percentage of cells with 2n DNA content and a significant increase in cells with DNA content between 2n and 4n. No significant change was observed in the percentage of cells with 4n DNA content. DNA content was measured 3 and 5 d post-transduction. D, MEF2D-depleted NRVMs showed no significant increase in cell size or markers of hypertrophy (E) 3 days post-transduction. Nppa, natriuretic peptide A (ANF); Nppb, natriuretic peptide B (BNP). The data are means ± S.E. (error bars). *, p < 0.05; **, p < 0.01.