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Degenerate CP1 domain endows hmtLeuRS insensitivity toward AN2690. A and B, aminoacylation by 300 nm hmtLeuRS and hmtLeuRS-EcCP1 in the presence and absence of 1 mm AN2690. C, effect of AN2690 on the post-transfer editing activities of hmtLeuRS and hmtLeuRS-EcCP1. 500 nm enzymes were used in the deacylation assay.
