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hmtLeuRS only possesses tRNA-independent pre-transfer editing activity. A, representative diagram of AMP formation assay conducted with 20 mm Nva by 3 μm hmtLeuRS in the absence and presence of 5 μm hmtRNALeu(CUN) and hmtRNALeu(UUR). B, corresponding graphical representations of the AMP formation rate using a TLC-based assay.
