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Mis-charging behavior of hmtLeuRS toward Val and Nva. A, representative diagram of aminoacylation assays using 300 nm hmtLeuRS in the presence of 5 μm mixture of 32P-labeled and -unlabeled EctRNALeu at 37 °C. The concentrations of Leu, Nva, and Val used here were 20, 20, and 100 mm, respectively. B, quantification of aminoacylated tRNA at various time points by hmtLeuRS for Leu, Nva, and Val.
