TABLE 1.

Kinetic parameters of hmtLeuRS, hmtLeuRS-ΔCP1, and hmtLeuRS-MmLinker toward Leu in amino acid activation

Data were obtained from three independent experiments. The catalytic efficiency of hmtLeuRS was designated as 100%.

	Km	kcat	kcat/Km	Relative
	μm	×10−2 s−1	s−1 mm−1	%
hmtLeuRS	30.4 ± 4.2	115 ± 25	37.8	100
hmtLeuRS-ΔCP1	19.3 ± 0.2	3.3 ± 0.4	1.7	4
hmtLeuRS-MmLinker	15.9 ± 0.2	12.1 ± 0.9	7.6	20