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Kinetic parameters of hmtLeuRS, hmtLeuRS-ΔCP1, and hmtLeuRS-MmLinker toward tRNALeu in aminoacylation
Data were obtained from three trials. The catalytic efficiency of hmtLeuRS was designated as 100%.
| Km | kcat | kcat/Km | Relative | |
|---|---|---|---|---|
| μm | ×10−2 s−1 | s−1 mm−1 | % | |
| hmtLeuRS | 2.14 ± 0.32 | 4.51 ± 0.24 | 21.0 | 100 |
| hmtLeuRS-ΔCP1 | 2.06 ± 0.22 | 0.40 ± 0.02 | 1.9 | 9 |
| hmtLeuRS-MmLinker | 1.65 ± 0.19 | 1.46 ± 0.26 | 8.8 | 42 |
