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. 2015 Aug 19;290(40):24413–24423. doi: 10.1074/jbc.M115.673012

FIGURE 3.

FIGURE 3.

Expression of Zfs1 target transcripts in the TZF domain complementation strains. Shown is a NanoString analysis of target transcripts in the S. pombe TZF domain Leu-1, zfs1Δ, and the complementation strains in which the S. pombe TZF domain was replaced with the indicated TZF domain. L1/L1R indicates the amino acid residue at the beginning of the TZF domain lead-in sequence (L/R)YKTE(P/L). Diagonally striped columns represent zfs1Δ, and dotted columns represent the L1R mutants. The y axes represent normalized RNA counts for the indicated transcript. Bm, B. mori; Cg, C. guilliermondii; Hs, H. sapiens; Sp, S. pombe. A—E, five of the 28 transcripts that were analyzed by NanoString. The y axes indicate normalized counts for the indicated transcripts, which are shown as the mean ± S.D. from at least four independent isolates. F, averages for all transcripts increased 2-fold or more in the zfs1Δ strain for the indicated substitution strains. These averages include 17 of the 28 transcripts from the NanoString analysis.