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. 2015 Aug 13;290(40):24424–24437. doi: 10.1074/jbc.M114.635672

FIGURE 2.

FIGURE 2.

Alignment of the P. aeruginosa CDO homologue with selected family members. Numbering of P. aeruginosa residues is shown at the top, and the extent of each sequence is indicated at the left. Residues of the CDO fingerprint proposed by Stipanuk et al. (23) are shown in white on black. White on gray indicates the possible divergence of the fingerprint in substrate specificity at position 62 in PA2602 (glutamine versus arginine) and the difference in uncross-linked residue at position 95 (glycine or alanine versus cysteine) and at position 168 (arginine versus various). Residues adjacent to and possibly influencing the cross-link are shown in black on light gray. The conserved cupin motif residues are indicated at the bottom. PA2602 from P. aeruginosa PAO1, NP_251292.1, is aligned with characterized 3MDOs (6) (V. paradoxus TBEA6, ACB72254; R. eutropha H16, YP_841375); CDOs (20, 21, 25) (Homo sapiens, NCBI protein sequence accession number BAA12873; R. norvegicus, BAA11925; Streptomyces coelicolor A3(2) (SCO3035), NP_627257; S. coelicolor A3(2) (SCO5772), NP_629897; B. subtilis, NP_390992; Bacillus cereus, NP_832375) and putative CDOs (23) with a putative cysteine-tyrosine cross-link (Vibrio splendidus 12B01, EAP93565.1; Chromobacterium violaceum ATCC, NP_902852.1).