TABLE 1.
Peptides identified by PRM and software-assisted spectrum interpretation using Mascot
| Predicted m/z (charge state)a | Peptide sequence identified by Mascot | Score | Modification |
|---|---|---|---|
| gi|372467082 chain A, crystal structure of cysteine dioxygenase from P. aeruginosa | |||
| 474.5772 (3+) | YQQYLLHVDSR | 36 | |
| 648.8433 (2+) | LEPGEVEALSPR | 85 | |
| 515.5866 (3+) | IGDVHQVSNAFSDR | 40 | |
| 829.4547 (2+) | TSISIHVYGANIGAVR | 48 | |
| 725.6833 (3+) | AVFSAEGEEKPFISGYSNSR | 70 | |
| gi|489228560 isoprenoid biosynthesis protein, partial (P. aeruginosa) | |||
| 755.7236 (3+) | LVTTPAYMLAQSIAEAASGINK | 85 | Oxidation (M) |
| No identification | |||
| 764.3578 (3+) | GAEYSQPYAFDAGGRPHPSGAR | ||
a Predicted m/z (charge state) is the m/z of the strongest charge state predicted by the Skyline software that was used to build the scheduled PRM assay. Score: Mascot ion score at a significance threshold of p < 0.05. An ion score of >56 indicates a significant peptide identification in a search against 48 million sequence entries of the non-redundant NCBI sequence database. All peptide sequences assigned to cysteine dioxygenase were unique for the identified protein.