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. 2015 Aug 17;290(40):24453–24469. doi: 10.1074/jbc.M115.665091

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

Author's Choice—Final version free via Creative Commons CC-BY license.

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FIGURE 4. — Role of SrtB in anchoring CD2831 to the cell wall. Supernatant (Sn), cell wall (CW), membrane (Mb), and cytosolic (Cy) compartment of zmpI mutant (ΔzmpI) and srtB/zmpI double mutant (ΔsrtBΔzmpI) strains, carrying pJKP041 (expresses CD2831 in the presence of ATc) and cultivated in the presence of 100 ng/ml ATc (+ATc) were analyzed by Western blotting with anti-CD2831 polyclonal antibodies. Supernatant and whole cell lysate (WL) fractions of ΔzmpI pJKP041 strain cultivated in the absence of ATc (−ATc) were also analyzed as negative controls.