FIGURE 6.
Structure of CD27L-released CD2831R-HA anchor peptides. Reverse phase-HPLC elution profile (A) and mass spectrometry spectrum (B) of CD2831R-HA anchor peptides. Purified CD2831R-HA was cleaved with Arg-C to generate C-terminal CD2831R-HA anchor peptides, which were further purified by a second round of anti-HA immunoprecipitation. Purified anchor peptides were analyzed by LC-MS. C, deduced structures and their calculated m/z value for the different species detected by mass spectrometry. D, MS/MS sequencing of the predominant ion at m/z 802.06 with z = 3. Inferred structure is represented. The indicated m/z values on the presented structure correspond to ions obtained by cleavage of one amide bond.