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. 2015 Aug 14;290(40):24484–24494. doi: 10.1074/jbc.M115.678243

FIGURE 5.

FIGURE 5.

Role of 15-lipoxygenase in IL-4-induced priming of LPS responses. A, mRNA expression of PPARγ, CD36, lipoprotein lipase, and FABP4 in cells treated with IL-4, AICAR, rosiglitazone, and phenformin for 48 h. *, p < 0.05 versus untreated cells. B and C, mRNA expression of IL-6, CXCL9, CXCL10, and IL-12p40 in cells treated with IL-4, AICAR, and phenformin (B) for 48 h, followed by medium change and 3-h treatment with LPS. C, cells were treated with nordihydroguaiaretic acid or PD146176 during the last 24 h of IL-4 treatment and LPS stimulation. D, mRNA expression of IL-6, CXCL9, CXCL10, and IL-12p40 in cells treated with LPS in the presence of 12(S)-HETE or 15(S)-HETE. *, p < 0.05 versus LPS. Data represent mean ± S.E. of at least three independent experiments. E, schematic of AMPK and ALOX15 effects on IL-4-induced macrophage priming. AA, arachidonic acid.