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. 2015 Aug 12;290(40):24495–24508. doi: 10.1074/jbc.M115.641944

FIGURE 3.

FIGURE 3.

Functional consequence of Ala substitution on Gq signaling of the conserved polar residues in the NK1 receptor. a, structure of the hydrogen bond network in the NK1 receptor model based on A2AAR (PDB code 4EIY) as a template structure. Key side chains are shown as sticks and water molecules as red spheres. b–h, agonist (SP)-induced IP3 production in COS-7 cells transiently transfected with either wild type NK1 (dotted lines) or mutant forms of ThrVII:13Ala (7.46) (b), GluII:10Ala (2.50) (c), AsnI:18Ala (1.50) (d), SerIII:15Ala (3.39) (e), SerVII:12Ala (7.45) (f), AsnVII:16Ala (7.49) (g), TrpVI:13Ala (6.48) (h), and TyrVII:20Ala (7.53) (i). Cell surface receptor expression measured by ELISA is shown in the column diagram insets in each panel.