FIGURE 5.
Functional consequence of Ala substitution on β-arrestin2 mobilization of the conserved polar residues in the NK1 receptor. a, structure of the hydrogen bond network in the NK1 receptor model based on A2AAR (PDB code 4EIY) as a template structure. Key side chains are shown as sticks and water molecules as red spheres. b–h, agonist (SP)-induced β-arrestin2 mobilization in CHOK1 cells transiently transfected with either wild type NK1 (dotted lines) or mutant forms of ThrVII:13Ala (7.46) (b), GluII:10Ala (2.50) (c), AsnI:18Ala (1.50) (d), SerIII:15Ala (3.39) (e), SerVII:12Ala (7.45) (f), AsnVII:16Ala (7.49) (g), TrpVI:13Ala (6.48) (h), and TyrVII:20Ala (7.53) (i).