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Functional consequence of Asp substitution of the GluII:10 (2.50) residue in the NK1 receptor. a–c, agonist (SP)-induced IP3 (a), cAMP (b), or β-Arrestin2 (c) mobilization in COS-7 cells (IP3 and cAMP) or CHOK1 cells (β-arrestin2) transiently transfected with either wild type NK1 (dotted lines) or mutant GluII:10Ala/Asp. Cell surface receptor expression measured by ELISA is shown in the column diagram inset in a.
