TABLE 2.
Ligand binding properties of SP to WT NK1 receptor and substitutions in position GluII:10 (2.50) and SerIII:15 (3.39) in the NK1 receptor using 125I-labeled SP as a radioligand
The constructs were expressed in transiently transfected COS-7 cells. Values are shown ± S.E.
| Construct | Kd (nm) |
|||||
|---|---|---|---|---|---|---|
| n | 0 mm NaCl | 50 mm NaCl | 100 mm NaCl | 200 mm NaCl | 300 mm NaCl | |
| WT NK1 | 5 | 0.125 ± 0.06 | 0.116 ± 0.03 | 0.187 ± 0.09 | 0.314 ± 0.16 | 0.253 ± 0.08 |
| GluII:10A (2.50) | 5 | 0.657 ± 0.28 | 0.520 ± 0.13 | 0.778 ± 0.11 | 1.45 ± 0.43 | 3.18 ± 2.09 |
| GluII:10D | 5 | 0.114 ± 0.04 | 0.128 ± 0.02 | 0.204 ± 0.06 | 0.290 ± 0.07 | 1.20 ± 0.54 |
| SerIII:15A (3.39) | 4 | 0.172 ± 0.04 | 0.469 ± 0.32 | 0.284 ± 0.13 | 0.310 ± 0.10 | 0.446 ± 0.09 |