FIGURE 5.

SiRNA silencing of HUWE1 and NEDD4-1 enhances ABCG1 efflux activity in CHO-K1 cells overexpressing ABCG1. A, ABCG1-mediated cholesterol efflux in CHO-K1 cells overexpressing ABCG1 after 6 h incubation with either 0.1% BSA alone or with addition of HDL₂ (10 μg/ml) as described under “Experimental Procedures.” Results are mean ± S.E. of three independent experiments, performed in triplicate cultures, with * indicating p < 0.05 compared with Control siRNA. B, ABCG1-mediated cholesterol efflux to HDL₂, expressed as fold change, relative to Control siRNA treatment. For each individual experiment, efflux to BSA was subtracted. Results are mean ± S.E. of three independent experiments, performed in triplicate cultures, with * indicating p < 0.05 compared with Control siRNA.