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SiRNA silencing of HUWE1 and NEDD4-1 in CHO-K1 cells overexpressing ABCG4. CHO-K1 cells stably overexpressing ABCG4 were transfected with control siRNA, siRNAs for HUWE1, or NEDD4-1 individually, or both for a total of 72 h as described under “Experimental Procedures.” Cells were harvested and cell proteins separated via SDS-PAGE, followed by immunoblotting with the indicated antibodies.
