TABLE 1.
List of yeast strains
All strains listed are W303 and derived from AFS92 (from A. Straight).
| Strain | Genotype |
|---|---|
| DOM918 | MAT a; PDS1-13XMyc:HIS3 |
| yJG2 | MAT a; PDS1-13XMyc:HIS3; pGAL-3XHA-UBC1:TRP1; UBC1-9XMYC::LEU2 |
| yJG3 | MAT a; PDS1-13XMyc:HIS3; pGAL-3XHA-UBC1:TRP1; ubc1ΔUBA-9XMYC::LEU2 |
| yJG5 | MAT a; PDS1-13XMyc:HIS3; pGAL-3XHA-UBC1:TRP1; empty vector::LEU2 |
| yJG10 | MAT a; PDS1-13XMyc:HIS3; UBC1-His61XFLAG:URA3(Kluyveromyces lactis) |
| yJG11 | MAT a; PDS1-13XMyc:HIS3; ubc1ΔUBA-His61XFLAG:URA3(K. lactis) |
| yJG14 | MAT a; PDS1-13XMyc:HIS3; pGAL-3XHA-UBC1:TRP1; ubc4Δ:LEU2; UBC1-9XMYC::LEU2 |
| yJG15 | MAT a; PDS1-13XMyc:HIS3; pGAL-3XHA-UBC1:TRP1; ubc4Δ:LEU2; ubc1ΔUBA-9XMYC::LEU2 |
| yJG16 | MAT a; PDS1-13XMyc:HIS3; pGAL-3XHA-UBC1:TRP1; ubc4Δ:LEU2; empty vector::LEU2 |
| yJG81 | MAT α; PDS1-13XMyc:HIS3; UBC4-UBA-9XMyc:LEU2 |
| yJG82 | MAT a; PDS1-13XMyc:HIS3; UBC4-UBA-9XMyc:LEU2 |
| yJG85 | MAT a; PDS1-13XMyc:HIS3; pGAL-3XHA-UBC1:TRP1; UBC1-9XMYC::LEU2; UBC4-UBA-9XMyc:LEU2 |
| yJG86 | MAT α; PDS1-13XMyc:HIS3; pGAL-3XHA-UBC1:TRP1; UBC1-9XMYC::LEU2; UBC4-UBA-9XMyc:LEU2 |
| yJG87 | MAT a; PDS1-13XMyc:HIS3; pGAL-3XHA-UBC1:TRP1; ubc1ΔUBA-9XMYC::LEU2; UBC4-UBA-9XMyc:LEU2 |
| yJG88 | MAT α; PDS1-13XMyc:HIS3; pGAL-3XHA-UBC1:TRP1; ubc1ΔUBA-9XMYC::LEU2; UBC4-UBA-9XMyc:LEU2 |