Table 1.
Primer sequences of genes selected for qPCR analysis
| Symbol | Gene title | PCR Primers | Sequences | Reference ID | Affymetrix ID |
|---|---|---|---|---|---|
| Actb | Actin, beta, cytoplasmic | Forward Reverse |
TTGCTGACAGGATGCAGAAG AGTCCGCCTAGAAGCACTTG |
NM_007393 | M12481_3_at |
| Cyr61 | Cysteine rich protein 61 | Forward Reverse |
TGGACAACCAGTGTACAGCA GATGCGGGCAGTTGTAGTTA |
BM202770 | 1438133_a_at |
| Egr1 | Early growth response 1 | Forward Reverse |
TCACCCACCATGGACAACTA AGCGGCCAGTATAGGTGATG |
NM_007913 | 1417065_at |
| Fos | FBJ osteosarcoma oncogene | Forward Reverse |
GAATGGTGAAGACCGTGTCA TGGGCTGCCAAAATAAACTC |
AV026617 | 1423100_at |
| Gem | GTP binding protein | Forward Reverse |
TAGTGCGGTGTCGAGAAGTG GACAGGTCATGGCAGGATTT |
U10551 | 1426063_a_at |
| H19 | H19 fetal liver mRNA | Forward Reverse |
AATGGTGCTACCCAGCTCAT GCAGAGTTGGCCATGAAGAT |
NM_023123 | 1448194_a_at |
| Igj | Immunoglobulin joining chain | Forward Reverse |
CCCTTTGAACAACAGGGAGA AGGTCTCAGGAACACCATCG |
BC006026 | 1424305_at |
| Mt1 | Metallothionein 1 | Forward Reverse |
CACCAGATCTCGGAATGGAC TGTCCCCAACAGAGAAGACC |
BC027262 | 1451612_at |
| Ntn1 | Netrin 1 | Forward Reverse |
TCCAAAGGCAAGCTGAAGAT GGCATTACCCAACAGCAAGT |
BI143915 | 1454974_at |
| Serpine1 | Serine (or cysteine) peptidase inhibitor, clade E, member 1 | Forward Reverse |
CCGATCCTTTCTCTTTGTGG GGATGCTGGTTGGAAAGATT |
NM_008871 | 1419149_at |
qPCR = Quantitative real-time polymerase chain reaction