Fig. 3. DHED treatment elicits neuroprotection in a rat model of ischemic stroke.

(A) OVX Sprague-Dawley rats were treated with DHED (0.8 to 100 μg/kg, s.c.) 1 h before tMCAO followed by 24-h reperfusion. The E2-treated group received 200 μg/kg, s.c. TCC-stained brain sections are representative of three animals per indicated treatment and from the same animals within each column. Pale-colored regions indicate areas of infarct whereas red-colored regions representing viable areas. Infarct volumes are charted as averages ± SEM (n = 3 per treatment group). (B) ND scores observed in the experiment described in (A). Data are averages ±SEM (n = 3 per treatment group). (C) Measured initial DHED to E2 conversion rates in major brain areas with tissue harvested 1 h after tMCAO. Data are averages ± SEM (n = 3 per area). (D) Infarct volumes after treatment with 100 μg/kg s.c. DHED 1 h, 2 h and 3 h after tMCAO. Data are averages ± SEM (n = 3 per treatment group). (E) ND scores observed in the experiment described in (D). P values were determined by one-way ANOVA using SNK multiple comparison test.