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. 2015 Oct 2;10(10):e0126943. doi: 10.1371/journal.pone.0126943

Table 1. List of oligonucleotide probes used for the detection of polymorphism in Pfdhfr and Pfdhps genes.

Gene Amino acid Probe sequences Hybridization Temperature
Pfdhfr Ser 108 5'-AACAAGCTGCGAAAGCATTCCAA-3' 54°C
Asn 108 5'-AACAAACTGGGAAAACATTCCAA-3' 54°C
Ile 51 5'-CCATGGAAATGTATTTCGCTAG-3' 45°C
Asn 51 5'-CCATGGAAATGTAATTCGCTAG-3' 54°C
Arg 59 5'-GAAATATTTTCGTGCAGTTAC-3' 48°C
Cys 59 5'-GAAATATTTTTGTGCAGTTAC-3' 58°C
Pfdhps Gly 437 5'-GAATCTTCTGGTCCTTTT-3' 43°C
Ala 437 5'-GAATCCTCTGCTCCTTTT-3' 51°C
Lys 540 5'-CAATGGATAAACTAACAA-3' 35°C
Glu 540 5'-CAATGGATGAACTAACAA-3' 35°C