Table 1. List of oligonucleotide probes used for the detection of polymorphism in Pfdhfr and Pfdhps genes.
Gene | Amino acid | Probe sequences | Hybridization Temperature |
---|---|---|---|
Pfdhfr | Ser 108 | 5'-AACAAGCTGCGAAAGCATTCCAA-3' | 54°C |
Asn 108 | 5'-AACAAACTGGGAAAACATTCCAA-3' | 54°C | |
Ile 51 | 5'-CCATGGAAATGTATTTCGCTAG-3' | 45°C | |
Asn 51 | 5'-CCATGGAAATGTAATTCGCTAG-3' | 54°C | |
Arg 59 | 5'-GAAATATTTTCGTGCAGTTAC-3' | 48°C | |
Cys 59 | 5'-GAAATATTTTTGTGCAGTTAC-3' | 58°C | |
Pfdhps | Gly 437 | 5'-GAATCTTCTGGTCCTTTT-3' | 43°C |
Ala 437 | 5'-GAATCCTCTGCTCCTTTT-3' | 51°C | |
Lys 540 | 5'-CAATGGATAAACTAACAA-3' | 35°C | |
Glu 540 | 5'-CAATGGATGAACTAACAA-3' | 35°C |