Fig 5. Amino acid alignment of chicken GLUT12 amino acid sequence with human GLUT12 sequence.

Alignment was performed using Clustalw algorithm. Amino acids are represented by the single letter code. Gaps in the amino acid sequences are indicated with a dash (-).* indicates positions which have a single, fully conserved residue. Lines with numbers indicate the 12 transmembrane domains (TM). (▼) indicates the predicted N-glycosylation sites. Other important conserved residues and motifs such as those corresponding to specific sugar transporter signatures are highlighted in blue boxes. NH2 and COOH terminal dileucine motifs are highlighted in green boxes. In the human amino acid sequence, the red box corresponds to the human peptide used to produce the anti-GLUT12 antibody.