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. 2015 Sep 28;10:6009–6020. doi: 10.2147/IJN.S85790

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© 2015 Lin et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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The uptake of BP/LPPC complex in DBTRG-05MG cells.

Notes: (A) BP or (B) BP/LPPC (50 µg/mL) was added to DBTRG-05MG cells and incubated for 15 minutes, 30 minutes, 60 minutes, and 120 minutes. Cell fluorescence was analyzed by confocal microscopy at 400×. PI red fluorescence: nuclei; blue fluorescence: BP drug; DiO green fluorescence: cell membrane. (C) The amount of BP in the cells. After treatment with BP, the cells were harvested and lysed. BP was extracted and measured by a fluorescence spectrophotometer. (D) Colocalization of BP/LPPC. BP/LPPC was added to DBTRG-05MG cells and incubated for 15 minutes and 60 minutes. Cell fluorescence was analyzed by confocal microscopy at 400×. DiO green fluorescence: DiO-labeled LPPC; blue fluorescence: BP drug; LysoTracker red fluorescence: lysosome.

Abbreviations: BP, n-butylidenephthalide; LPPC, lipo-PEG-PEI complex; PI, propidium iodide.

The uptake of BP/LPPC complex in DBTRG-05MG cells.

Notes: (A) BP or (B) BP/LPPC (50 µg/mL) was added to DBTRG-05MG cells and incubated for 15 minutes, 30 minutes, 60 minutes, and 120 minutes. Cell fluorescence was analyzed by confocal microscopy at 400×. PI red fluorescence: nuclei; blue fluorescence: BP drug; DiO green fluorescence: cell membrane. (C) The amount of BP in the cells. After treatment with BP, the cells were harvested and lysed. BP was extracted and measured by a fluorescence spectrophotometer. (D) Colocalization of BP/LPPC. BP/LPPC was added to DBTRG-05MG cells and incubated for 15 minutes and 60 minutes. Cell fluorescence was analyzed by confocal microscopy at 400×. DiO green fluorescence: DiO-labeled LPPC; blue fluorescence: BP drug; LysoTracker red fluorescence: lysosome.

Abbreviations: BP, n-butylidenephthalide; LPPC, lipo-PEG-PEI complex; PI, propidium iodide.