Table 1.

The cytotoxicity of BP/LPPC (IC₅₀) in different brain tumor and normal cells

Cell line	Tumor type	BPa	BP/LPPCb	BP/Lipo	Folda/b
Brain tumor cells
DBTRG-05MG	Human GBM	54.9±2.8c	11.6±3.4d	71.4±6.0e	4.72
G5T/VGH	Human GBM	55.8±1.9c	12.1±1.9d	94.7±3.2e	4.62
GBM8401	Human GBM	108.5±0.8c	17.5±1.5d	145.7±1.3e	6.22
GBM8901	Human GBM	122.7±2.3c	14.4±0.3d	151.2±0.5e	8.54
RG2	Rat GBM	57.6±2.6c	11.9±0.1d	72.1±2.3e	4.84
N18	Mouse neuroblastoma	58.3±1.4c	13.1±1.5d	118.7±3.2e	4.46
Normal cells
SVEC	Mouse endothelial cell	106.9±1.0c	24.3±2.1d	110.5±2.9c	4.40
MDCK	Dog normal kidney	131.5±3.6c	32.2±1.6d	169.0±3.0c	4.08

Notes: All values are mean ± standard deviation (n=3, units of BP concentration: µg/mL). Values in the same row with different superscript letters are significantly different at P<0.05.

^a/b(IC₅₀ of BP)/(IC₅₀ of BP/LPPC).

^c–eValues in the same row with different superscript letters are significantly different at P<0.05.

Abbreviations: BP, n-butylidenephthalide; GBM, glioblastoma multiforme; LPPC, lipo-PEG-PEI complex; IC₅₀, half maximal inhibitory concentration.