Table 1.
Bacterial strains and plasmids used in this studya
| Bacterial strain or plasmid | Description | Reference, Source |
| S. oneidensis strains | ||
| MR-1 | Manganese-reducing strain from Lake Oneida, N.Y. sediments | [1] |
| OMP35-1 | omp35 mutant derived from MR-1; omp35::Kmr | This work |
| OMCA-1 | omcA mutant derived from MR-1; omcA::Kmr | [8] |
| OMCB-1 | omcB mutant derived from MR-1; omcB::Kmr | [8] |
| ETRA-153 | etrA mutant derived from MR-1; etrA::Kmr | [17] |
| CMA-1 | menaquinone-minus mutant derived from MR-1 | [6] |
| E. coli strains | ||
| JM109 | recA1 F' traD36 lacIq Δ(lacZ)M15 proA+B+/ e14-(McrA-) Δ(lac-proAB) thi gyrA96 (Nalr) endA1 hsdR17 (rK- mK+) relA1 supE44 | [61] |
| S17-1λpir | λ(pir) hsdR pro thi; chromosomally integrated RP4-2 Tc::Mu Km::Tn7; donor strain to mate pEP185.2-derived plasmids into MR-1 | [62] |
| S17-1 | C600::RP-4 2-Tc::Mu-Km::Tn7 hsdR hsdM recA thi pro; donor strain to mate pVK100- derived plasmids into MR-1 | [62] |
| TOP10 | F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Smr) endA1nupG; used as host for plasmids derived from pCR2.1-TOPO | Invitrogen |
| Plasmidsb | ||
| pBCSK | 3.4-kb vector; pUC ori; Cmr; used for complementation | Stratagene |
| pBComp218F & R | pBCSK with the MR-1 omp35 open reading frame plus associated 5' and 3' regions; 218 bp upstream sequence in the forward (F) and reverse (R) orientation | This work |
| pBComp411F & R | pBCSK with the MR-1 omp35 open reading frame plus associated 5' and 3' regions; 411 bp upstream sequence in F and R orientation | This work |
| pCR2.1-TOPO | 3.9-kb vector for cloning PCR products; Kmr Apr | Invitrogen |
| pUT/mini-Tn5Km | Apr; Tn5-based delivery plasmid; used as source of Kmr gene | [63] |
| pTOPO/omp35 | pCR2.1-TOPO with the MR-1 omp35 open reading frame plus associated 5' and 3' regions; 5.6 kb | This work |
| pTOPO/omp35:Km | pTOPO/omp35 with the Kmr gene replacing bases 458–850 of omp35; 7.3 kb | This work |
| pEP185.2 | 4.28-kb mobilizable suicide vector derived from pEP184; oriR6K mobRP4 Cmr | [64] |
| pDSEPomp35 | Kmr-interrupted omp35 gene cloned into the XhoI site of pEP185.2; Cmr Kmr; 7.7 kb; used for gene replacement to generate strain OMP35-1 | This work |
aNalr, Smr, Kmr, Apr, Tcr, and Cmr, resistance to nalidixic acid, streptomycin, kanamycin, ampicillin, tetracycline, and chloramphenicol, respectively. bThe vector pBCSK freely replicates in MR-1 while pEP185.2 does not.