Extended Data Table 1. Statistical comparison for orientation bias and resection in Sγ1 and Sε junctions from WT and DSBR-deficient B cell libraries.
Unpaired two-tailed t-test for the degree of bias in the Sγ1 (a) and Sε (b) junctions between WT and DSBR-deficient B cells with full-length Sμ was performed for experiments described in Figs. 3 and 4. Orientation bias was calculated as described in methods for individual libraries. Numbers in parenthesis indicate times of independent experiments performed for each genotype. N/A: not available. Unpaired two-tailed t-test for the level of resections in the Sγ1 (c) and Sε (d) junctions was performed for experiments described above. Percentage of resection was calculated as described in Methods for individual libraries. Numbers in parenthesis indicate number of times of independent experiments performed for each genotype. N/A: not available.
| Extended Data Table 1a Statistical analysis of bias ratio of Sγ1 junctions | ||||||
|---|---|---|---|---|---|---|
| WT | ATM−/− | H2AX−/− | 53BP1−/− | 53BP1−/−+ Ai | Rif1−/− | |
| WT (n=5) | ||||||
| ATM−/− (n=3) | <0.0001 | |||||
| H2AX−/− (n=3) | 0.0057 | 0.0031 | ||||
| 53BP1−/− (n=8) | <0.0001 | <0.0001 | <0.0001 | |||
| 53BP1−/−+Ai(n=3) | <0.0001 | 0.0039 | 0.001 | 0.1481 | ||
| Rif1−/− (n=3) | <0.0001 | 0.181 | 0.0094 | <0.0001 | 0.0029 | |
| Extended Data Table 1b Statistical analysis of bias ratio of Sε junctions | ||||||
|---|---|---|---|---|---|---|
| WT | ATM−/− | H2AX−/− | 53BP1−/− | 53BP1−/−+ Ai | Rif1−/− | |
| WT (n=5) | ||||||
| ATM−/− (n=3) | <0.0001 | |||||
| H2AX−/− (n=3) | 0.0034 | 0.0291 | ||||
| 53BP1−/− (n=5) | <0.0001 | 0.0003 | 0.0002 | |||
| 53BP1−/−+Ai(n=3) | N/A | N/A | N/A | N/A | ||
| Rif1−/− (n=3) | 0.0012 | 0.9274 | 0.1154 | 0.0003 | N/A | |
| Extended Data Table 1c Statistical analysis of resection of Sγ1 junctions | ||||||
|---|---|---|---|---|---|---|
| WT | ATM−/− | H2AX−/− | 53BP1−/− | 53BP1−/−+ Ai | Rif1−/− | |
| WT (n=5) | ||||||
| ATM−/− (n=3) | 0.0002 | |||||
| H2AX−/− (n=3) | 0.0037 | 0.3012 | ||||
| 53BP1−/− (n=8) | <0.0001 | 0.0002 | 0.0008 | |||
| 53BP1−/−+Ai(n=3) | <0.0001 | 0.158 | 0.7974 | 0.0005 | ||
| Rif1−/− (n=3) | N/A | N/A | N/A | N/A | N/A | |
| Extended Data Table 1d Statistical analysis of resection of Sε junctions | ||||||
|---|---|---|---|---|---|---|
| WT | ATM−/− | H2AX−/− | 53BP1−/− | 53BP1−/−+ Ai | Rif1−/− | |
| WT (n=5) | ||||||
| ATM−/− (n=3) | <0.0001 | |||||
| H2AX−/− (n=3) | <0.0001 | 0.0279 | ||||
| 53BP1−/− (n=5) | <0.0001 | 0.0001 | 0.0003 | |||
| 53BP1−/−+Ai(n=3) | N/A | N/A | N/A | N/A | ||
| Rif1−/− (n=3) | <0.0001 | 0.0199 | 0.058 | 0.0062 | N/A | |