Table 2.

Primers used for amplification and cloning of the phosphatase cDNAs

cDNA cloned	Primer pair
PfCnA	N6CTCGAGGAACCACTGCCTGATCCGAAG
	N6GGATCCTTATTCGTTGGATGGCCTCTTTTCGT
PfCnB	N6GCTAGCATGGGAAACACACAAGCGATATTATC
	N6CTCGAGTAATTCTAGCTTCAATTTATTTCCAAC
PfPP7	N6CTCGAGGAAAATTACAACATTGAAGATGTTG
	N6GGATCCTTAATTATTTGAATATATATATGTAGGC

In each pair, the first primer is the 5' sense primer, and second one is the 3' antisense primer. All primers contained a random hexanucleotide sequence (N6) for efficient restriction of the PCR product, followed by restriction sites (underlined): XhoI (CTCGAG), BamHI (GGATCC), NheI (GCTAGC). All sequences are written 5' → 3'. Note that the natural start codons (ATG) of both PfCnA and PfPP7 were removed because translation would originate from the upstream ATG of the pET-15b vector (in order to add the N-terminal His-tag). In PfCnB, the ATG was left intact but the stop codon was removed so that translation could continue into the pET-23a vector to add the C-terminal His-tag.