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. 2015 Oct 2;10(10):e0138059. doi: 10.1371/journal.pone.0138059

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© 2015 Li et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — (A) In vitro BrdU assay performed on Axin2 ^-/- and wild type FOb and POb cells undergoing differentiation reveals increased proliferative activity in Axin2 ^-/- cells than corresponding wild type. (B) in vivo PCNA immunostaining performed on coronal sections derived from frontal and parietal bones of pN21 Axin2 ^-/- and wild type mice, also indicates an increase in proliferation of Axin2 ^-/- frontal and parietal bones compared wild type. (C) Quantification of PCNA staining obtained by calculating the percentage of PCNA positive cells over the total cell number counted at least in five equivalent areas of each bone, indicates the lowest cell proliferation activity in wild type parietal bone, whereas Axin2 ^-/- parietal bone displays activity similar to that of wild type frontal bone. Scale bar = 150 μm.