Skip to main content
. 2015 Oct 2;11(10):e1005196. doi: 10.1371/journal.ppat.1005196

Fig 5. Cytosolic AS2 is a functional PTGS suppressor.

Fig 5

(A-G) Suppressor activity of AS2, AS2-NES, AS2-NLS and AS2 I88A mutant. N. benthamiana (line 16c) leaves were co-infiltrated with 35S:GFP and 35S:AS2 or 35S:AS2-NES or 35S:AS2-NLS or 35S:AS2I88A (local silencing, A-D). E-G show systemic silencing. AS2 can interfere with systemic silencing induced by 35S:GFP (compare 5F of 35S:AS2 co-infiltrated with 5E of mock co-infiltrated with 35S:GFP and 5G with AS2 I88A mutant). Bar = 10 mm. Bar = 10 mm. (H) Western blot analysis showing GFP protein levels in N. benthamiana leaf (line 16c) infiltrated with 35S:GFP along with 35S:AS2, 35S:AS2-NES, 35S:AS2-NLS, 35S:AS2I88A, or mock treatment (upper panel). Coomassie blue-staining of the large subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase (rbcL) is shown as a loading control (lower panel). (I) Western blot analysis showing AS2 and its variants protein levels in N. benthamiana leaf. Coomassie blue-staining of the large subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase (rbcL) is shown as a loading control (lower panel).